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Mouse FAP culture, osteogenic differentiation, and quantification FAPs were isolated from nave hamstring muscles as described above (CD3, CD45R/B220, CD11b, Gr1, Ter119)-negative CD45 CD31 Sca1 + CD34 + ) and were sorted directly into MEM with 20% FCS, and subsequently seeded into T25 flasks in DMEM with high glucose (GIBCO Invitrogen, Cat# 11960-044) containing 20% (v/v) FCS, 10% horse serum, 10 U penicillin/mL, 10ug/mL streptomycin and 2 mM l-glutamine