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10.1016/j.biopsych.2008.03.004 Biol Psychiatry
Whilst it is not yet licensed for routine clinical use, it has generated significant interest because it targets three separate hormone receptors , rather than one or two as current weight loss medications do
The lysis buffer was prepared by mixing 10 ml of M-PER (ThermoFisher) with 100 l of Protease & Phosphatase inhibitor mix (ThermoScientific) and 100 l of 0.5 M EDTA (ThermoScientific)
[12] [13] Endogenous GLP-1 has a very short half-life, typically 12 minutes, as it is rapidly degraded by the enzyme dipeptidyl peptidase-4 (DPP-4)
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