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A pilot metabolic study demonstrated that a 750 mg NAD+ IV infusion over 6 hours was welltolerated in humans, with rapid metabolic clearance and no acute toxicity [10]

Cancer Cell 23, 811825

Briefly, the cells were incubated with 200 L culture medium containing 15 L PCG-MSNs-H 1 H 2 H 3 probes for different times, then were washed with 200 L hybridization buffer (pH 7.4) once, MTT (0.5 mg mL 1 , 100 L) were seeded in the wells and incubated at 37 C for 4 h