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The culture was split into two 300 ml cultures and each flask was diluted to 1.0 10 6 cells/mL, usually leading to a final volume of 600 mL that complies with the ratio of air to cell medium of 4:1
RNA extraction and qRTPCR analysis Cells were grown to 80% confluency in 75 cm 2 flasks, harvested by trypsinisation, counted using a haemocytometer, and 1 10 7 cells used for RNA extraction using a Qiagen RNeasy mini kit (Qiagen), following the manufacturers protocol for mammalian cells, with additional on column DNAse digestion (RNAse free DNAse kit, Qiagen)
Role in Hair and Scalp Formulations GHK-Cu also supports scalp health and follicle activity
