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This improvement is attributed to increased GLP-1 activity and direct inhibition of DPP-4 expressed in the liver [119]
After a wash in TBS for 10 min, slides were incubated for 45 min in Flex+Linker mouse (Dako) or Post Primary (Leica, Zrich, Switzerland), then washed again in TBS, and incubated for 45 min in Flex+HRP (Dako) or Polymer (Leica)
The key is keeping intensity low enough that you finish feeling refreshed rather than depleted
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