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The obstacle was that native GLP-1 is cleaved by dipeptidyl peptidase-4 between its second and third residues almost immediately, leaving a circulating half-life measured in a couple of minutes
Glucose uptake in muscle 3 H-2-deoxyglucose (DG) uptake into muscle tissue was evaluated as previously described 83 , with the exception that the bound radiolabeled compound was separated from free radiolabel by washing the tissue multiple times in radiolabel-free medium
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