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-arrestin 2 recruitment HEK293T cells (3.2 10 6 cells/10 cm plate) were grown for 24 h before transiently transfection with 15 g plasmid containing GIPR, GLP-1R or GCGR tagged with Rluc8 and -arrestin 2 with a Venus-tag at the N terminus at a ratio of 1:9 by Lipofectamine 3000 transfection reagent
Biochimie 201:717 Peng X, Zhou C, Hou X, Liu Y, Wang Z, Peng X, Zhang Z, Wang R, Kong D (2018) Molecular characterization and bioactivity evaluation of two novel bombinin peptides from the skin secretion of Oriental fire-bellied toad, Bombina orientalis
