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You would never know by looking at it, because the solution might still appear clear, but the biological activity would be degraded
Protein precipitation was performed by adding 10 volumes of 25% MeOH in acetonitrile (ACN), except for the brain homogenate which was extracted in 100% MeOH
Strategies to mitigate these side effects include starting with a lower dose and gradually increasing it to improve tolerance
Proper injection technique ensures that you get the most benefit from your treatment
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