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ZincHomocysteine binding in cobalamin-dependent methionine synthase and its role in the substrate activation: DFT, ONIOM, and QM/MM molecular dynamics studies
Agonist dissociation was monitored immediately after washing in 10 M exendin(9-39) by TR-FRET at 37 C, with 50 s delay and 300 s integration time, and binding quantified as 520 nm over 620 nm signal after nonspecific binding subtraction with excess (10 M) unlabeled agonist
As a strong antioxidant, vitamin E helps keep cells and tissues safe from the damage caused by oxidative stress (48)
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