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Consider that immediate can range from a few hours to a day post-injection, depending on the individuals metabolism and overall health

For DIA LCMS analysis, 250 ng of peptides from each sample were injected into an HPLC (nanoElute, Bruker Daltonics) and loaded onto a trap column with a constant flow of 4 L/min (Acclaim PepMap100 C18 column, 0.3 mm id 5 mm, Dionex Corporation) then eluted onto an analytical C18 Column (1.9 m beads size, 75 m 25 cm, PepSep) heated at 50 C

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I started doing the protocol with Day Spring that the Doctor associated with Day Spring suggested