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To assess bacterial degradation of heavy GSH, we pretreated AGS cells with 50 g/mL [2- 13 C 1 ]-Gly for 24 h, then washed the cells to remove exogenous [2- 13 C 1 ]-Gly prior to infection with WT, gGT , or gGT :: gGT H

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Do I reconstitute all 10mg at one time, or dose by dose