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Rare hypersensitivity reactions , including cobalt allergy (as cobalamin contains cobalt), have been documented
selected an optimized n-hexane-ethyl acetate-methanol-water (3:7:4:6, v/v) solvent system for countercurrent chromatography based on partition coefficients (k) and separation factors (), utilizing the lower phase as mobile phase in head-to-tail elution mode, where 400 mg crude extract yielded pure senkyunolide-I (6.4 mg, 98%) and senkyunolide-H (1.7 mg, 93%) in a single run (32)
275 For example, more mtDNA mutations were observed in aged oocytes than in young ones, and the increased mutations inhibited the ovarian function, resulting in fewer available follicles and affecting female fertility
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