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Following dissociation, islets cells were washed 3 times with PBS containing 0.01% BSA, followed by centrifugation at 352 g for 5 minutes, and then resuspended in PBS containing 0.01% BSA at a concentration of approximately 250,000 cells/mL, which was used for downstream DROP-Seq and DART-Seq experiments
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Research protocols typically run 14-21 day cycles
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