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Under cysteine-limiting conditions, Glu is recycled into Glu (a futile cycle) via 5-oxoprolinase at the expense of two ATP molecules without GSH production and this is the reason for the decrease in GSH synthesis as well as the depletion of ATP in these cells [14]
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NADPH, generated through glutamine metabolism, supports glutathione regeneration, helping keep ROS levels in check
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