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cDNA was amplified using a real-time PCR machine (QuantStudio, Thermo Fisher Scientific) with primers shown in Table S9
Sermorelin works by supporting the bodys own natural growth hormone production, and each persons hormonal function, metabolism, sleep quality, activity level, and medical history are unique
The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)
10.1007/s12519-018-0210-2 8
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