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The cells were then collected by centrifugation, washed with cold PBS and incubated with annexinV-Pacific Blue in annexin-binding buffer at 20 C for 15 min
Quantitative proteome analysis of ovarian cancer tissues using a iTRAQ approach
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Other interesting observations come from primary sclerosing cholangitis: specific strains of Klebsiella pneumonia detected in these patients could damage gut barrier integrity, favoring bacterial translocation of other pathobionts toward the mesenteric lymph nodes, and prime a TH17 cell response in the liver, if transplanted into mice models [46]
