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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol
Methods: We performed single-cell RNA sequencing on 33 kidney samples, constructing an atlas comprising 82,661 cells
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Glutathione injections are typically administered intramuscularly or intravenously, depending on the desired effect and treatment protocol
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