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Biochemical determinations For human studies, serum levels of human FGF21, and plasma levels of OXM, adiponectin, GLP-1, insulin and glucagon were analyzed in a subsample of patients included in each surgical group by ELISA immunoassay according to the instructions of manufacturer
Palmitate oxidation rates were detected at 28 C using two media as already described 59 , the first media allowing both mitochondrial and peroxisomal -oxidation to occur, and the second one allowing peroxisomal -oxidation only
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