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Used pens should be returned to a community pharmacy for safe disposal, and needles disposed of in designated sharps containers
GPX4 is eventually reactivated by a second glutathione molecule, releasing glutathione disulfide (GS-SG)
The pre-dosing solution was subsequently discarded and replaced with 50 L of the probe substrate and compounds or known inhibitor mixture incubated for 10 min at 37C
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