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To obtain bacteriological sterility prior to conducting immunopeptidomics experiments, the cell pellet was lysed using 2x lysis buffer and complete protease inhibitor cocktail (Roche) for 30 minutes on ice, with periodic shaking every 10 minutes

Because those identified antigens resulted from specific HLA alleles in THP-1 cells, we expanded the screening of T cell epitopes using the entire antigen sequence against the most frequent HLA alleles worldwide to maximize population coverage, which is important for vaccine development

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