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Furthermore, it was shown that iron in compounds such as ferrioxamine B, ferrichrome, ferritin, Imferon, cytochrome c, FePO 4 , and [Fe(OH)3]n was not readily available to N

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For the co-immunoprecipitation (co-IP) process, 50 L of magnetic beads (Protein G, Invitrogen, #10003D) were prepared by resuspending them in a tube, separating them from the solution using a magnet, and washing them with NT2 buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM MgCl, 0.05% NP-40)

Day-to-day consistency matters more than the exact hour, and you should confirm any routine with your provider