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The lysate was cleared by centrifugation and the protein was purified by Ni-His-affinity chromatography in batch mode
Authors contributions Drafting the manuscript, creating figures and tables (WMW), and critically revising the document for important intellectual content (LGL)
This can result in loss of strength and physical function, which may affect balance, mobility, and the ability to perform everyday activities
But the data suggest that this success is conditional
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