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Transwell chambers were assayed for cell invasion ability by applying 100 L of DMEM-diluted Matrigel gel (3:7 dilution) to the chambers to be checked and incubated at 37 C for 30 min to form a gel, then pre-treated MIA PaCa-2 and PANC-1 cells were resuspended with DMEM and spread in small chambers at 50000/well, and the lower chamber was placed in 20% FBS DMEM, incubated at 37C 5% CO 2 for 48 hours

[1] [2] Common adverse effects include gastrointestinal symptoms such as nausea, vomiting, diarrhea, and constipation, which typically diminish with continued use

The first preparative solution phase synthesis of Melanotan II. Beilstein Journal of Organic Chemistry vol