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Using a converted boiler as a hyperbaric chamber in Kansas City, Missouri (altitude 909 ft), Cunningham replicated this improvement caused by altitude descent on human disease when he treated an agonal Spanish flu patient in 1918, achieving complete recovery with four daily 1-h 1.6 ATA hyperbaric air treatment (HBAT) sessions (6, 7) Countless Spanish flu patients followed, and Cunningham extended his clinical treatment benefits to a variety of conditions (79)
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Preparation methods (including solvent selection, concentrations, and handling steps) should follow validated institutional SOPs appropriate to the assay system and analytical objectives
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