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Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection
Glutathione levels also decrease as we age and our need for supplemental glutathione increases significantly
Mistake 5: using too much or too little water Adding more water than intended (say, 3ml instead of 2ml) dilutes the solution beyond your planned concentration
600 mg per vial lets a provider adjust up or down based on labs and tolerance
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