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Delayed Centrifugation Weakens the In Vitro Biological Properties of Platelet-Rich Fibrin Membranes. Clinical Oral Investigations , 2024, Salgado-Peralvo et al., Medicina 2023 Integrative Review Medicina 2023 Integrative Review Understanding Solid-Based Platelet-Rich Fibrin Matrices in Oral and Maxillofacial Surgery: An Integrative Review Results This review pulls together timing data (including Castros experiments) and states that: PRF tubes should ideally be centrifuged within 90 seconds to avoid diffuse fibrin polymerization