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To address the reactivities of the redox-sensitive cysteinyl residues, we also analyzed the recombinant monothiol roGFP2 variants roGFP2 C151S and roGFP2 C208S and detected small but reproducible glutathionylation-dependent changes of fluorescence at both excitation wavelengths for roGFP2 C151S (SSG) and roGFP2 C208S (SSG) (Fig

Then, the fluorescent signal was recorded at 527 nm in the absence of mitochondria (maximal signal) and monitored over time after adding the mitochondria suspension, until the equilibrium was reached (maximal quenching)

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Polyamines interact with phytohormone signalling (ABA, auxin), calcium signalling and metabolic networks