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Here we determined the rate constants for the individual redox reactions between PfGrx, a model glutaredoxin from Plasmodium falciparum , and redox-sensitive green fluorescent protein 2 (roGFP2), a model substrate and versatile tool for intracellular redox measurements
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To identify potential off-target substrates, we searched enriched motifs against the human proteome, using the enrichment score of the IL-1 motif (cleavage between Glu 18 and Asp 19) as the cutoff for predicted cleavage activity (Fig
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