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Sourcing and preparation Standard tirzepatide formulations (Mounjaro, Zepbound) come in pre-filled pens designed for specific dose increments
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Brain tissue was homogenised with 1:10 (w/v) PBS on ice, then centrifuged at 3,000 rpm for 15 min at 4 C in an Eppendorf centrifuge and the supernatant was collected
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