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For replacement experiments, isogenic HeLa cell lines were induced with tetracycline overnight prior to siRNA transfection
Taken together, these studies establish potentially converging disease mechanisms in ALS and spinal muscular atrophy, with ALS-causative mutants acquiring properties representing both gain (dysregulation of SMN) and loss (reduced RNA processing mediated by U1-snRNP) of function
Gearhardt, A
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