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1 B, C, E ) and amplitudes to a smaller degree (Fig
Briefly, for each single TIMS (100 ms) in diaPASEF mode, we used 1 mobility window consisting of 27 mass steps (m/z between 114 and 1414 with a mass width of 50 Da) per cycle (1.27 s duty cycle)
In earlier work, Sekhar's group had shown that in healthy adults, the levels of oxidative stress, oxidative damage and glutathione remain stable until people enter their 60s when oxidative stress and oxidative damage begin to increase and glutathione to decline
Previous work has shown that the abundance of Lactobacillus reuteri (normalized to a control group) decreases during the onset of obesity and remains suppressed even after dieting in mice 99 , which parallels our observations that vehicle-treated diet-switched mice had lower levels of Lactobacillus than the 5A-1MQ-treated mice, the latter of which also demonstrated greater weight loss
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