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To understand a bit more what Hydrolyzed Collagen is, you have to know that proteins are large chains of amino acids connected with so-called peptide bonds

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For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method

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