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Complete media consisted of DMEM (Wisent, 319-005-CL) supplemented with 2% FBS (Wisent, 080-150), 5 ng/ml EGF (Winsent, 511-110-UM), 1 g/ml Hydrocortisone (Sigma, H4001), 5 g/ml Insulin (Wisent, 511-016-UG), 35 g/ml Bovine Pituitary Extract (BPE Hammond CellTech, 1078-NZ), 25 g/ml Amphotericin B (Wisent, 450-105-QL), 50 g/ml Gentamycin Sulfate (Wisent, 450-135-XL) and 50g/ml Penicillin/Streptomycin (Wisent, 450-200-EL)
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Some users taking oral BPC-157 above 1000 mcg daily report increased fatigue or mild cognitive changes, likely related to the peptide's effects on neurotransmitter systems
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