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The cells were blocked with 5% BSA and then incubated with primary antibodies overnight, followed by incubation with FITC, goat anti-mouse IgG (A22110), DyLight 594, or goat anti-rabbit IgG for 1 h

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In animal models, a modulatory effect has also been observed at the level of the carotid sinus, suggesting that this pharmacological class may influence sympathetic tone regulation during hyperglycemic states (47)