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The column was washed with 10 mL of binding buffer, followed by 10 mL of wash buffer, before eluting the bound proteins using 3 mL of elution buffer

However, decades later, many questions remain unanswered

2015 American thyroid association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: the American thyroid association guidelines task force on thyroid nodules and differentiated thyroid cancer