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428 The early mechanistic models of MAPK pathway dynamics by Doug Lauffenburger quantified how negative feedback controls signal adaptation, and his concept of temporal collateral sensitivity provides a theoretical basis for adaptive therapy

Theoretical concerns are not proof of harm, but they identify areas off-target SAM effects, signalling-pathway impacts, and uncharacterised interactions that require study before any conclusion about human safety could be drawn

Supernatants were transferred into a new tube containing Laemmli buffer and 2-mercaptoethanol

These effects are believed to be related to the broader systemic benefits of optimized GH circulation on brain function and hormonal balance