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Optimal grids for AMYR:Cagri complexes and CTR: Cagri ( N = 1) were imaged by conventional cryo-TEM on a Thermo Fisher Scientific Titan Krios microscope operated at an accelerating voltage of 300 kV with a 50 m C2 aperture and a 100 m objective aperture at an indicated magnification of 105kx in nanoprobe EFTEM mode, yielding a calibrated pixel sampling of 0.85 .pix -1
Brains were removed, post-fixed in the same fixative for 1 wk, washed 3 times with PBS, and then stored in PBS at 4C
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For example, a syringe may be listed as 1 mL, 30G, 1/2 inch, U-100, which means the listing shows barrel capacity, needle gauge, needle length, and unit-of-use marking format at the same time
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