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Hormones, metabolism, sleep quality, stress, insulin resistance, thyroid function, medications, and nutrient deficiencies can all play a role in how the body gains, stores, and loses weight

Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

This results in the inhibition or reduction of the synthesis of the target protein

Over time, this may help reduce hyperpigmentation, sun spots, melasma, and even overall skin tone