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The membranes were washed with TBST three times each for 10 min
Affinity cell sorting enriches transfection-positive cells Then we transfected Lenti-X 293T and K-562 cells with the six variants separately and performed affinity cell isolation using GSH magnetic beads 30 h post-transfection
When exposed to oxygen, two glutathione molecules bond through their sulfur atoms, forming GSSG
These results demonstrated the additive benefits of targeting both amylin and GLP-1 pathways for glycemic control and weight management in type 2 diabetes and supported advancement to Phase 3 development
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