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Vigorous shaking creates foam, which means air is being forced into the solution, and the mechanical stress of bubble formation can denature the peptide
Final Word If it sounds too good to be true it probably is
A 2022 paper in the International Journal of Molecular Sciences characterised effects on proliferative activity and inflammatory pathways in monocyte/macrophage THP-1 cell research (PMID 35408963)
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