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In summary, future research directions should shift from an abstract technical checklist to clearly defined testable hypotheses centered around PCDimmune pathology: using spatial and single-cell technologies to answer does PCD geography determine pathological phenotypes, characterizing how death patterns dynamically switch during acute exacerbation periods through in vivo imaging, and leveraging genetic epidemiology to analyze can PCD executor polymorphisms define clinical internal phenotypes. On this basis, multi-omics and AI can truly become the bridge connecting basic mechanisms, immune internal phenotypes, and clinical trial designs, and targeted interventions based on PCD can move from the conceptual level to operable precise immunotherapy strategies ( Figure 10 )
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After successful infusion and removal of the pancreas from the mouse, pancreata were further incubated in a 50 mL Falcon tube with the collagenase solution for 1012 min at 37 C in a water bath
Enhanced Passive Ca2+ Reabsorption and Reduced Mg2+ Channel Abundance Explains Thiazide-Induced Hypocalciuria and Hypomagnesemia
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