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Allowing the vial to come to room temperature for about 15-20 minutes before adding the bacteriostatic water can help prevent pressure changes inside the vial and ensure the powder dissolves more readily
This leads to the mediation of the transcription of NLRP3 and pro-IL-1, resulting in the production of many inactive inflammatory mediators, leading to subsequent protease cleavage and activation ( FA can also induce the direct activation of the NLRP3 inflammasome in macrophages
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