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Protein extraction and digestion for mass spectrometry For protein extraction and digestion, we used the in-strip protein digestion method, as previously described 21

Here are a few simple tips: Use the lower belly or upper thigh as injection sites Always clean the skin first with an alcohol wipe Switch locations each time to avoid soreness Use a fresh, clean needle for every dose Dont inject into areas that already feel tender or irritated If youre not sure if youre doing it right, were happy to walk you through the process or even demo it for you in person

A sudden dark black spot on lip may result from trauma, a blood blister, allergic reaction, or sun exposure