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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min
The flow rate of the mobile phase was 0.5 ml/min
4 C,E ), consistent with the glutathione cycle being able to compensate for decreased availability of glutamine, an established source of glutamate neurotransmitter
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