Despite the improvement in GH/IGF-1 levels (GH 10.6 ng/mL, IGF-1 462 ng/mL), her glycemic control drastically deteriorated for 4 months (Hb1Ac 8.212.0%)
Methylcobalamin is the active form of vitamin B12 and can be readily utilised by the body without conversion
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Membrane was blocked in 5% (w/v) non-fat dry milk in TBST for 1 h at room temperature, and probed against the anti-glutathione antibody (Virogen, Grater Boston, MA, USA) at the dilution of 11000 in 1% (w/v) non-fat dry milk TTBS over night at 4C, and secondary antibody, goat anti-mouse IgG (Calbiochem, San Diego, CA, USA) conjugated to horseradish peroxidase, at 17500 in 1% (w/v) non-fat dry milk for 1 h at room temperature