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Zhong Y, Zhong P, He S, Zhang Y, Tang L, Ling Y, et al
The VP0, VP1, and VP3 fragments of the optimized PV1 wtP1 gene were amplified by PCR and cloned into the pPinK-HC vector (Invitrogen) using ClonExpress Ultra One Step Cloning kit (Vazyme, China), yielding plasmids HC-wtVP0, HC-wtVP1, and HC-wtVP3, respectively
Furthermore, building upon the existing Modified Multiple Platform Method (MMPM) animal model, our research seeks to develop a dual-factor model that integrates sleep and dietary factors (by integrating HFD, ADF, and MMPM) to more accurately reflect clinical pathogenesis, and to investigate whether this model results in disruptions to energy metabolism and oxidative stress-induced brain damage in model rats
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