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This method allowed the goodness of fit to inform upon an appropriate model structure when the actual reaction rates were unknown and, in doing so, provided a useful indication of the possible mechanism by which this process occurs
For a 70 mg vial, draw 3.0 mL
The PCR product containing the polymorphism rs2070593 was digested with 7 U of Psp CI restriction enzyme (Sibenzyme, Novosibirsk, Russia) producing two fragments of 183 and 180 bp (these bands overlap each other at electrophoresis) in case of the G allele and leaving the A allele uncut (one fragment of 363 bp) on 2%-agarose gel
Process Biochem 42:454458
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