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Use within the supplier-stated beyond-use window

Before conjugation, 1 mL of Labelling Buffer (8 mM Na 2 HPO 4 , 137 mM NaCl, 1.47 mM KH 2 PO 4 and 2.68 mM KCl, pH 8.0) containing 20 M GK was mixed with 1 L of FITC-NHS dye (100 M, prepared from a 100 mM stock solution dissolved in anhydrous DMSO)

Each one reduces efficacy, wastes product, or introduces unnecessary risk