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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing
10.1016/s0021-9673(03)00434-5 J
Use with caution in pregnant animals
Perturbation of glutathione homeostasis sensitized hepatocytes to TNF-induced cell death TNF plays an important role in the progression of NAFLD
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