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The mice were randomly divided into eight groups: control, vehicle, positive control (0.15 g/kg silymarin), glutathione (0.0166 g/kg), Ganoderma lucidum (Gan, 0.0666 g/kg), vitamin C (0.0666 g/kg), and GGV groups at two doses (0.15 g/kg, 0.45 g/kg)

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Role of mitochondria and cardiolipins in growth inhibition of breast cancer cells by retinoic acid

The samples are centrifuged (5,000 rpm, 5 min), 30 L of the resulting supernatant and 30 L of the Ellmans reagent are added to 1 mL of phosphate buffer (pH 7.8), and the optical density is measured at =412 nm and =700 nm [19]